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WP6: Measurement and Analysis of Phenotypic and Anatomic Traits

The phenotypic and anatomic adaptation traits in *Cedrus brevifolia* will be assessed by examining:

(i) **Needles **

Morphological and anatomical measurements will be done using needles in their second or third year of growth. The assessed morphological features of needles are: **N1:** number of needles per rosette, **N2:** length of needles, **N3:** width in the middle of the needle, **N4:** maximum width of the needle,** N5**: ratio of length to width in the middle of the needle, **N6:** ratio of length to maximum width of the needle, **N7:** needle length, width in the middle of the needle, and ratio of length to width, for each auxiblast needle, **N8:** sharpness of needles.

In addition, the anatomical features of needles that will be compared biometrically are: **NA1:** dimension of stomata chambers, **NA2:** width of cuticle, **NA3:** number of stomata per unit area, **NA4:** number of rows of stomata and **NA5:** number of resin canals.

(ii) **Cones** **C1:** cone length, **C2:** diameter of cone in the middle, **C3:** maximum cone diameter, **C4:** ratio of cone length to diameter of cone in the middle, **C5:** ratio of cone length to maximum diameter**, C6:** length of the stalks of cones and **C7:** number of seeds per cone.

(iii)** Seeds **

**S1:** Total seed-**S2:** seed length, **S3:** width in the middle of the seed, **S4:** maximum width of the seed, **S5:** ratio of length to width in the middle of the seed, **S6**: ratio of length to maximum width of the seed, **S7:** wing length, **S8:** width in the middle of the wing, **S9:** maximum width of the wing, **S10:** ratio of length to width in the middle of the wing and **S11:** ratio of length to maximum width of the wing.

Statistical analysis of these traits will be done using various statistic methods (i.e. minimum, maximum and mean values, standard error, coefficient of variation, ANOVA, hierarchical cluster analysis, principal components analysis, Euclidean distance). These procedures will assist in extracting conclusions for the phenotypic and anatomic traits divergence among the subpopulations. This data will also be a powerful tool as an evidence of the species adaptation at local patterns.

The deliverable (D) of this WP is:*D15: Preliminary report with the results from the statistical analysis of phenotypic and anatomic traits.*